Evaluation of Connexin 43 Redistribution and Endocytosis in Astrocytes Subjected to Ischemia/Reperfusion or Oxygen-Glucose Deprivation and Reoxygenation

Figure 2

Representative confocal images of Cx43 distribution under different conditions at 6 h after reperfusion (red, Cx43; green, GFAP; blue, Hoechst 33342; magnification was 400x, resp.). (a) Under normal cultivation conditions, Cx43 was mainly distributed in the cytoplasmic membrane, and its density was higher in the adjacent cell membrane joint. A small amount of scattered Cx43 has been circulated in the cytoplasm with a few particles distributed in the nucleus. (b) Under IR/OGDR conditions with physiological saline, Cx43 was distributed in the membrane, cytoplasm, and nucleus, with the highest content in the cytoplasm. These vesicles of different sizes and shapes were observed in the cytoplasm. The total number of particles of this group was almost the same as that of the normal group (), but the cytoplasm particles of this group were significantly more than those of the normal group (). (c) Under IR/OGDR conditions with CBX intervention, Cx43 was distributed in the membrane, cytoplasm, and nucleus but mainly in the cytoplasm. There was no significant difference between the CBX intervention and saline control group ().

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