Structure and Function of Trypsin-Loaded Fibrinolytic Liposomes

Figure 7

Lipid ester carbonyl stretching band region for empty (SSL) and trypsin-loaded () liposomes. The solid black lines are the measured spectra; the blue and the red lines are the fitted bands corresponding to intact and H-bonded C=O groups, respectively. The green band component can be attributed to residual water (after subtraction) and to amide I band positions for curve fitting using the second derivative; band shapes were approximated by Lorentzian functions. The intensities and the bandwidth of each component were optimized according to a minimization procedure. The relative contribution of each of the particular components was calculated from their integrated areas in the best fit.

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