Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico

Figure 5

HDAC6 protein overexpression and its effect on tubulin acetylation in HEK 293T cells. HEK293T cells were transfected with either the empty vector (mock transfection) or pLVX-EF1α-hdac6–IRES-zSGREEN and then processed for either indirect immunofluorescence detection of HDAC6 expression or western blot analysis. A significantly increased expression of HDAC6 was observed in the HDAC6-transfected cells (a-left panel). Quantification of HDAC6 expression was done by Image J analysis. Graphical presentation of the same is seen (a-right panel). Values are mean ± SEM. . The transfected cell lysates were electrophoresed on 10% SDS-PAGE and transblotted for detection of acetyl α-tubulin and α-tubulin using their respective antibodies (b). The blot is a representative image of 3 independent experiments.

(a)

(b)