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BioMed Research International
Volume 2017, Article ID 5232680, 5 pages
Research Article

Kinetics of Tyrosinase Inhibitory Activity Using Vitis vinifera Leaf Extracts

1Department of Chemical Engineering, National United University, Miaoli, Taiwan
2Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan
3Department of Applied Cosmetology, Master Program of Cosmetic Science, Hung-Kuang University, Taichung, Taiwan

Correspondence should be addressed to Wen-Ying Huang;

Received 20 February 2017; Revised 13 April 2017; Accepted 30 April 2017; Published 1 June 2017

Academic Editor: Mohamed Trigui

Copyright © 2017 Yung-Sheng Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Natural medical plant is considered as a good source of tyrosinase inhibitors. Red vine leaf extract (RVLE) can be applied to a wide variety of medical disciplines, such as treatments for chronic venous insufficiency over many decades. This study investigated the tyrosinase inhibitory activity of RVLE containing gallic acid, chlorogenic acid, epicatechin, rutin, and resveratrol which are effective for skin hyperpigmentation. The five components contents are 1.03, 0.2, 18.55, 6.45, and 0.48 mg/g for gallic acid, chlorogenic acid, epicatechin, rutin, and resveratrol. The kinetic study showed the tyrosinase inhibitory of RVLE via a competitive reaction mechanism. RVLE solution has an IC50 (the half inhibitory concentration) value of 3.84 mg/mL for tyrosinase inhibition, that is, an effective tyrosinase inhibitory activity, and can be used as a whitening agent for cosmetic formulations in the future.