Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells

Figure 2

YAP decreases PTEN expression via miR-29a. (a) The PTEN expression at the mRNA level is similar between the three groups. Mean ± SD (). (b) Western blot analysis of PTEN expression shows that miR-29a OE group can decrease the PTEN and miR-29a KD group can increase it. (c) There is the quantification of densitometric levels of PTEN. Mean ± SD (). An ANOVA test is used here. , . (d, f) miR-29a mediates the expression of the PTEN protein. YAP overexpression and miR-29a inhibitor have increased protein levels of PTEN and decreased p-Akt. YAP knockdown and miR-29a mimic have decreased PTEN expression and increased p-Akt. There is no change of Akt protein. (e, g) There are the quantification of densitometric levels of PTEN, Akt, and p-Akt. Mean ± SD (). Student’s -test is used here. .

(a)

(b)

(c)

(d)

(e)

(f)

(g)