Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells

Figure 3

YAP promotes the neurite outgrowth of N2a cells. (a) GAP-43 and NF-200 expression are measured in N2a cells. Representative immunofluorescence is observed with anti-GAP-43 and anti-NF-200 treated with YAP (B, D) and control (A, C). The blank vector is used as a control. Mean ± SD (). Scale bar = 50 μm. (b, c) Western blot analysis shows the protein expression of GAP-43 and NF-200 for treatment group and control group. There are obvious differences in neural protein expression. (d, e) There is the quantification of densitometric levels of GAP-43 and NF-200. Mean ± SD (). Student’s -test is used here. . (f, g) Real-time PCR indicates that YAP group expresses NF-200 and GAP-43 higher than control group. Mean ± SD (). Student’s -test is used here .

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