Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells

Figure 8

The results of a ChIP assay. (a) It is the 2% agarose gel electrophoresis with the different sonication conditions. It is 25% power, working 2 seconds, 2.5 seconds, and 1.5 seconds in sequence and pausing 8 seconds, and total time is 3 minutes. The leftmost is the marker which contains 2000 bp, 1000 bp, 750 bp, 500 bp, 200 bp, and 100 bp from top to bottom. (b) The picture exhibits the most appropriate method which is working 2.5 seconds and pausing 8 second and the total time is 3 minutes. The chromosome segment is mainly focused on from 200 bp to 500 bp. (c) The results of agarose gel electrophoresis of the samples are showed which is performed with semiquantitative PCR. From left to right, there are the marker (100 bp), the YAP antibody group (binding site 2), the RNA polymerase II antibody group, the normal IgG group, and the input group. (d) The chart reveals the quantification PCR of the samples. The result reveals that YAP binds the promoter of the miR-29a and the major binding site is the binding site 2. Mean ± SD (). An ANOVA test is used here. .

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