Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression

Table 1

Primer sequences used for PCR and real-time RT PCR.

Gene	ID	Primer sequence (5′-3′)		Product size (bp)
GAPDH	NM_001206359	Forward	GTCGGTTGTGGATCTGACCT	124
		Reverse	TTGACGAAGTGGTCGTTGAG
shTNFRI-Fc	NC_000006.12	Forward	ATA AGCTTATGGGCCTCTCCACCGTGC	633
		Reverse	ATCTCGAGTCATGTGGTGCCTGAGTCCTC
HO-1	NC_000022.11	Forward	ATGGAGCGTCCGCAACCCGACAG	867
		Reverse	TCACATGGCATAAAGCCCTACAG
HO-1	NM_002133.2	Forward	TCTCTTGGCTGGCTTCCTTA	109
		Reverse	ATTGCCTGGATGTGCTTTTC
Ferritin heavy chain	NM_213975.1	Forward	ATGAGCAGGTGAAAGCCATC	110
		Reverse	CAGGGTGTGCTTGTCAAAGA
Ferritin light chain	NM_001244131.1	Forward	GGAGCGTCTCTTGAAAATGC	125
		Reverse	CCATAGCGTCCTGGGTTTTA
Transferrin	NM_001244653.1	Forward	GCCACGGAAACCTATTGAGA	126
		Reverse	ACACTGTTGGCACAGTTTGG
iNOS	NM_001143690.1	Forward	CCACCAGACGAGCTTCTACC	113
		Reverse	TCCTTTGTTACCGCTTCCAC

rimers for PCR.