Overhydroxylation of Lysine of Collagen Increases Uterine Fibroids Proliferation: Roles of Lysyl Hydroxylases, Lysyl Oxidases, and Matrix Metalloproteinases

Figure 1

(a) Hydroxyproline levels, as a measure of total collagen, in fibroid tissues and normal myometrial tissues (): tissue samples were air-dried and weighed and 100 mg of the tissues was subjected to acid hydrolysis in 6 N HCl solution for 16 h at 110°C. The hydrolysates were suspended in an equal volume of 6 N NaOH and filtered. The concentration of hydroxyproline was determined according to Woessner’s method. Hydroxyproline concentration was expressed as μg per mg dry tissue weight. (b) The levels of total, soluble, and insoluble (cross-linked) collagen were determined in samples of UF and the adjacent normal myometrial tissues. Aliquots of tissue extracts (50 μl) were used to determine the collagen contents and the extent of collagen cross-linking as described under Materials and Methods. Collagen concentration was expressed as μg per mg dry tissue weight. (c) The ratio of insoluble:soluble forms of collagen in samples of UF and normal myometrial tissues. Values represent the means ± SEM (). Significant difference at .

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