Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography

Figure 1

Fabrication and observation of layered human iPS cell-derived cardiac cell sheet-tissues using centrifugation. A detached human iPS cell-derived cardiac cell sheet was transferred onto a polystyrene culture dish (a). The culture dish with the cell sheet was centrifuged (23 ×g, 3 min), and the cross-sectional observation was performed by optical coherence tomography (OCT) (b and c). After incubation (37°C, 5 min), another cardiac cell sheet was transferred onto the first cardiac cell sheet (d). Multilayered cardiac cell sheets were centrifuged (12 ×g for 1 sec and again after removal of the extra medium 34 ×g for 3 min); then the cross-sectional observation of the cell sheets was performed (e and f).