Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography

Figure 3

Optical coherence tomography (OCT) observation of double-layered human iPS cell-derived cardiac cell sheets fabricated by a conventional method. Just after the layering of a human iPS cell-derived cardiac cell sheet onto first cardiac cell sheet, there were significant spaces between the double-layered cell sheets (a). In the conventional method without centrifugation, some spaces between the double-layered cardiac cell sheets were detected even after the 15 min incubation (b), and spaces were hardly detected after the 30 min incubation (c). Three experiments were performed independently and all of them showed similar results. An OCT system (IVS-2000) was used in this study.