MicroRNA-31 Function as a Suppressor Was Regulated by Epigenetic Mechanisms in Gastric Cancer

Figure 3

miR-31 induced early apoptosis in gastric cancer cell. (a, b) 48 hours after transfection of NC.miR or miR-31 mimics, apoptosis assay was assessed to determine apoptosis rate of BGC-823 and SGC-7901 cells. (c) The total RNA was extracted from transfected cells after 24 hours, and the mRNA level of caspase 3 was measured by real-time PCR. β-Actin was an internal control. (d) The total protein was extracted from transfected cells with RIPA solution after 48 hours, and caspase 3 protein was detected by western blotting. β-Actin was an internal control ).

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