MicroRNA-31 Function as a Suppressor Was Regulated by Epigenetic Mechanisms in Gastric Cancer

Figure 8

miR-31 regulated HDAC2 expression in gastric cancer. (a) Wild type psi-CHECK2-HDAC2-3′-UTR-wt plasmid was cotransfected with miR-31 mimics into BGC-823 and SGC-7901 cells. Relative luciferase activity was detected using dual luciferase system. The mRNA relative expression (b) and protein level (c) of HDAC2 were detected in BGC-823 and SGC-7901 cells which was transfected with miR-31 mimics or NC.miR. Wild type or mutant HDAC2 3′-UTR plasmid was cotransfected with miR-31 mimics or NC.miR in BGC-823 (d) and SGC-7901 cells (e). Relative luciferase activity was detected. (, , ns, no significance).

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