Significance of Heavy-Ion Beam Irradiation-Induced Avermectin B1a Production by Engineered Streptomyces avermitilis

Figure 1

Growth kinetics of the original strain and the mutant strains AO-M₃1, HS-P₁8, and 147-G₅8 cultivated with shaking. (a) Time course of the cell dry weight and dextrin consumption of the original strain and the mutant strains AO-M₃1, HS-P₁8, and 147-G₅8. Left panel, comparison of the AO-M₃1 mutant (black circles and black triangles) with original strain S. avermitilis (white circles and white triangles). Middle panel, comparison of the 147-G₅8 mutant (black circles and black triangles) with original strain S. avermitilis (white circles and white triangles). Right panel, comparison of the HS-P₁8 mutant (black circles and black triangles) with original strain S. avermitilis (white circles and white triangles). (b) Time profiles of the AVM B1a specific productivity of original strain and AO-M₃1, HS-P₁8, and 147-G₅8 mutant. On the left is comparison of AO-M₃1 mutant (black box) with the original strain S. avermitilis (white box), the comparison of 147-G₅8 mutant (black box) with the original strains S. avermitilis (white box) is in the middle, and on the right is comparison of HS-P₁8 mutant (black box) with the original strains avermitilis (white box). Data are the average of quintuplicate samples; the error bars indicate the standard deviations. All results were analyzed with Tukey’s test (duplicate determinations, ).

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