Comparison of DNA Methylation in Schwann Cells before and after Peripheral Nerve Injury in Rats

Figure 2

(a) The shape of activated Schwann cells (ASCs) under optical microscope. (b) The shape of normal Schwann cells (NSCs) under optical microscope. Both of these SCs, long spindle cells, were arranged in fish shape and nucleus was ovoid or oblong. Scale bar: 200 um. (c) SCs were marked with S-100ß by immunofluorescence. (d) The nucleus of SCs was marked with DAPI by immunofluorescence. (e) SCs and nucleus of SCs were merged together by immunofluorescence. Both of the two kinds of SCs had positive results under fluorescence microscope. Scale bar: 100 um. (f) The growth rate of SCs was showed by optical density (OD) value of 450 nm from 1 day to 7 days through cells proliferation assay. The growth rate of ASCs was obviously higher than that of NSCs starting from 2 days.