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BioMed Research International
Volume 2017 (2017), Article ID 5404682, 9 pages
https://doi.org/10.1155/2017/5404682
Research Article

Analysis of Differentially Expressed Genes in Gastrocnemius Muscle between DGAT1 Transgenic Mice and Wild-Type Mice

1Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
2The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China

Correspondence should be addressed to Bo Zuo; nc.ude.uazh.liam@obouz

Received 28 October 2016; Accepted 22 January 2017; Published 13 March 2017

Academic Editor: Young-Mi Lee

Copyright © 2017 Fei Ying et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Fig S1 Identified by polymerase chain reaction(PCR)-based genotyping F1 generation of TG mice. M maker DL2000 1,2,5 transgenic mice, 3,4,6 WT mice, N negative control.

Fig S2 The result of RNA gel electrophoresis. C1,C2,C3 WT mice, C4,C5,C6 transgenic mice.

Fig S3 The result of chip signal value distribution. The X-axis represents the signal of the probe, the Y-axis represents the number of probe. The different color is the different sample(red, deep blue and light blue are transgenic mice; green, purple, orange are WT mice). 1-28-1, 1-28-5 and 1601-1-1 are the transgenic mice; 3YCY1, 3YCY2, 3YCY3 are the wild-type mice.

Fig S4 The result of principal component analysis. The different color of the ball is the different sample (red, deep blue and light blue are transgenic mice; green, purple, orange are WT mice). The purple cycle is transgenic group and the yellow cycle is WT group. 1-28-1, 1-28-5 and 1601-1-1 are the transgenic mice; 3YCY1, 3YCY2, 3YCY3 are the wild-type mice.

Fig S5 Hierarchical cluster result of differential expressed genes. The X-axis represents the probe, the Y-axis represents the sample(red, orange, deep blue are transgenic mice and yellow, green, light blue are WT mice). The different of the color means the distance of the cluster. 1-28-1, 1-28-5 and 1601-1-1 are the transgenic mice; 3YCY1, 3YCY2, 3YCY3 are the wild-type mice.

Table S1 43 up-regulated and 28 down-regulated genes in the microarray analysis.

  1. Supplementary Material