BioMed Research International / 2017 / Article / Tab 4

Research Article

Susceptibility of In Vitro Melanoma Skin Cancer to Photoactivated Hypericin versus Aluminium(III) Phthalocyanine Chloride Tetrasulphonate

Table 4

The Annexin V FITC apoptosis detection kit was performed to evaluate mode of cell death on A375 cells after treatment with Hyp-PDT or AlPcS4Cl-PDT.

VariableMode of cell death
Live cells (%)Necrotic cells (%)Early apoptotic cells (%)Late apoptotic cells (%)

Cells only100000
Cells + 5 J/cm2 at 594 nm92.722.72.6
Cell + Hyp (2.5 µM)
Cells + PDT 4 hrs53.51.424.520.6
Cells + PDT 24 hrs251.645.128.3

Cells only100000
Cells + 5 J/cm2 at 682 nm92.
Cells + AlPcS4Cl (2.5 µM)
Cells + PDT 4 hrs81.
Cells + PDT 24 hrs74.53.918.23.4

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