Research Article
Susceptibility of In Vitro Melanoma Skin Cancer to Photoactivated Hypericin versus Aluminium(III) Phthalocyanine Chloride Tetrasulphonate
Table 4
The Annexin V FITC apoptosis detection kit was performed to evaluate mode of cell death on A375 cells after treatment with Hyp-PDT or AlPcS4Cl-PDT.
| | Variable | Mode of cell death | | Live cells (%) | Necrotic cells (%) | Early apoptotic cells (%) | Late apoptotic cells (%) |
| | Hyp | | Cells only | 100 | 0 | 0 | 0 | | Cells + 5 J/cm2 at 594 nm | 92.7 | 2 | 2.7 | 2.6 | | Cell + Hyp (2.5 µM) | 93.7 | 0.4 | 4.2 | 1.7 | | Cells + PDT 4 hrs | 53.5 | 1.4 | 24.5 | 20.6 | | Cells + PDT 24 hrs | 25 | 1.6 | 45.1 | 28.3 |
| | AlPcS4Cl | | Cells only | 100 | 0 | 0 | 0 | | Cells + 5 J/cm2 at 682 nm | 92.7 | 1.5 | 3.1 | 2.7 | | Cells + AlPcS4Cl (2.5 µM) | 87.5 | 0.4 | 9.9 | 2.2 | | Cells + PDT 4 hrs | 81.3 | 1.2 | 14.3 | 3.1 | | Cells + PDT 24 hrs | 74.5 | 3.9 | 18.2 | 3.4 |
|
|
