Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion

Figure 1

RT-QuIC data. (a) A representative RT-QuIC response. A set of RT-QuIC quadruple reactions was performed with mouse rPrP (89–231) and PrP seeds from ScN2a cells diluted 5 × 10⁻⁴-fold. RT-QuIC was performed using a modified version of the method described by Atarashi et al. [21]. The average ThT fluorescence was plotted against assay time. (b) Differences in length of lag phase. (c) Differences in amyloid formation rate. (d) Differences in ThT fluorescence maxima. (e) Differences in integrated area under the curve. Averages and standard deviations (error bars) were calculated using multiple data sets (). Student’s -test was used for statistical analysis. A value < 0.05 () was considered statistically significant.

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