Prion Diagnosis: Application of Real-Time Quaking-Induced Conversion

Table 1

Assays to measure PrP aggregation and seeding activity of PrP.

	Substrate	Denaturant	Detergent	Product	Detection method	Real-time detection
ASA/PAFA	Recombinant PrP (α-PrP)	Guanidine-HCl	No	Aggregates, amyloid	ThT fluorescence	Yes
PMCA	PrP from cells or tissues	No	Triton X-100	PrP	WB for PK-resistant PrP	No
RT-QuIC	Recombinant PrP (α-PrP)	No	SDS	Aggregates, amyloid	ThT fluorescence	Yes

α-PrP prepared from recombinant PrP with a high α-helical content. SDS: sodium dodecyl sulfate; WB: western blotting; PK: proteinase K; ThT: thioflavin T.