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BioMed Research International
Volume 2017, Article ID 5430723, 9 pages
https://doi.org/10.1155/2017/5430723
Research Article

Helicobacter pylori from Peptic Ulcer Patients in Uganda Is Highly Resistant to Clarithromycin and Fluoroquinolones: Results of the GenoType HelicoDR Test Directly Applied on Stool

1Department of Medical Microbiology, Makerere University College of Health Sciences, P.O. Box 7072, Kampala, Uganda
2Department of Midwifery, Faculty of Health Sciences, Lira University, P.O. Box 1035, Lira, Uganda
3Department of Medicine, Makerere University College of Health Sciences, P.O. Box 7072, Kampala, Uganda
4MBN Clinical Laboratories, Plot 28 Nakasero Road, Kampala, Uganda

Correspondence should be addressed to Freddie Bwanga; ude.esac@81bxf

Received 30 September 2016; Revised 13 January 2017; Accepted 13 February 2017; Published 7 May 2017

Academic Editor: Frederick Adzitey

Copyright © 2017 Denish Calmax Angol et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure 3 (supplementary materials) shows representative hybridization results of the GTHDT on the nitrocellulose strips as obtained in our laboratory. In strip (a) the H. pylori strain was sensitive to both FQL and CLA because of presence of probe bands in the gyrA and 23S rRNA wild type loci but no mutation bands. In strip (b) the H. pylori strain was resistant to both FQL and CLA because of presence of mutation bands in both the gyrA 91 (MUT1) and 23S (MUT3) probe loci. In strip (c) the H. pylori strain was resistant to FQL because of presence of mutation band in the gyrA 91 (MUT1) but sensitive to CLA because of presence of the 23S wildtype probe bands but no mutation bands in the 23S (MUT1-MUT3) probe loci. In strip (d) (negative control) only the conjugate and amplification control bands are seen and no other bands because only PCR water was tested.

Kit insert for the Taytec® Helicobacter pylori antigen test in stool.

  1. Supplementary Materials