Oxidative Stress Induced by Polymyxin E Is Involved in Rapid Killing of Paenibacillus polymyxa

Table 1

Primers used for gene amplification in the PCR.

	Forward (5′ - 3′)	Reverse sequence (5′ - 3′)
sodA	GGCATTTCAATTACCAGAAC	GCAGCCGCGTAACGTTTGTT
sodB	ATGCTGAGTACTTATGGGTCTTTCC	TTAGAAGGGTTGCCATCTCAGC
ilvD	GCGGTCCTATGAAAGCTGGT	CGTTACTTGCGTTCGTCACC
Fur	CCGTGGAAGTCCAAACGATG	GCAATCCCAGGGCTACAAAC
Dps	AACCGTCAAGTCGCTAACCT	CCCAAATAAGAGCGCAGCATC
16S rRNA	AGAGTTTGATCCTGGCTCAG	ACGGCTACCTTGTTACGACTT

sodA, sodB, ilvD, Fur, and Dps encode Mn-superoxide dismutase (Mn-SOD), Fe-superoxide dismutase (Fe-SOD), dihydroxy-acid dehydratase (DHAD), Fur-like protein, and DNA-binding proteins (Dps), respectively.
primers for different genes were designed based on the reference gene sequences of P. polymyxa E681 (GenBank accession number CP000154) and P. polymyxa SC2 (GenBank accession number CP002213), and the primers of 27F and 1492R were used for 16S rRNA gene.