Mechanism of Reduced Susceptibility to Fosfomycin in Escherichia coli Clinical Isolates

Table 1

PCR and real-time RT-PCR primers used in this study.

Primer		Sequence (5′-3′)	Reference
murA-full	F	AAACAGCAGACGGTCTATGG	[18]
	R	CCATGAGTTTATCGACAGAACG
glpT-full	F	GCGAGTCGCGAGTTTTCATTG	[18]
	R	GGCAAATATCCACTGGCACC
uhpT-full	F	TTTTTGAACGCCCAGACACC	[18]
	R	AGTCAGGGGCTATTTGATGG
uhpT-partial	F	ATGCTGGCTTTCTTAAACC	[19]
	R	TTATGCCACTGTCAACTGC
uhpA-full	F	GATCGCGGTGTTTTTTCAG	[18]
	R	GATACTCCACAGGCAAAACC
uhpA-partial	F	ATCACCGTTGCCCTTATAGA	[19]
	R	TCACCAGCCATCAAACAT
ptsI-full	F	GAAAGCGGTTGAACATCTGG	[18]
	R	TCCTTCTTGTCGTCGGAAAC
cyaA-full	F	AACCAGGCGCGAAAAGTGG	[18]
	R	ACCTTCTGGGATTTGCTGG
rpoD-qPCR	F	CAAGCCGTGGTCGGAAAA	[20]
	R	GGGCGCGATGCACTTCT
uhpT-qPCR	F	AAGCCGACCCTGGACCTT	[20]
	R	ACGGTTTGAACCACATTTTGC

Primers designated “full” were used for direct sequencing, and “qPCR” were used for real-time RT-PCR.