Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production

Figure 1

SDS-PAGE with corresponding zymogram of AmyMH expressed in Brevibacillus choshinensis SP3. Lane 1, molecular mass marker. Lane 2, protein from a culture supernatant of B. choshinensis SP3/pNCMO2. Lane 3, protein from a culture supernatant of B. choshinensis SP3/pNCMO2-AmyMH. Lane 4, purified samples of AmyMH. Lane 5, zymogram of protein from a culture supernatant of B. choshinensis SP3/pNCMO2. Lane 6, zymogram of the protein from a culture supernatant of B. choshinensis SP3/pNCMO2-AmyMH. Lane 7, zymogram of the purified AmyMH.