Research Article

Antineoplastic Effects of Honokiol on Melanoma

Figure 3

Effects of honokiol on DNA fragmentation by TUNEL assay and flow cytometry in SKMEL-2 (a) and UACC-62 (b) cells. Cells were treated with 0–100 μM honokiol for 24, 48, or 72 hours. After the respective treatments, TUNEL assay was performed by using APO-BrdU TUNEL assay kit. The extent of DNA fragmentation was quantified by computational analysis of cells staining positive for BrdU using CellQuest software. The bars indicates the percentages of apoptotic cells with fragmented DNA. indicates statistical significance in honokiol treated groups as compared to the control cells. .
(a)
(b)