XRRA1 Targets ATM/CHK1/2-Mediated DNA Repair in Colorectal Cancer

Figure 2

Downregulation of XRRA1 expression inhibits cell proliferation in HT29 and HCT116 cell lines. (a) MTT assay of HT29 and HCT116 cell proliferation after downregulation of XRRA1 expression by the sh-XRRA1 vector. (b) The percentages of cells incorporated by EdU were determined by examining at least 200 cells per sample in multiple fields. Data in C are averages of three repeats. (d) Cell cycle analysis by flow cytometry of XRRA1 at low expression in HT29 and HCT116 cells. Data in E are the averages of three repeats. (f) Western blot was used to determine the expression of cyclin E, p21, and cyclin A at the protein level after sh-XRRA1 was infected into HT29 and HCT116 cells. (g) Quantitative real-time PCR was used to determine the expression of cyclin E, p21, and cyclin A at the mRNA level after sh-XRRA1 was infected into HT29 and HCT116 cells.

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