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BioMed Research International
Volume 2017, Article ID 5746768, 8 pages
https://doi.org/10.1155/2017/5746768
Research Article

Pharmacokinetic and Toxicological Evaluation of a Zinc Gluconate-Based Chemical Sterilant Using In Vitro and In Silico Approaches

1Department of Biophysics and Biometry, Rio de Janeiro State University, Boulevard 28 de Setembro, 87 Fundos, 4° Andar, 20551-030 Rio de Janeiro, RJ, Brazil
2Department of Genetics and Molecular Biology, Federal University of the State of Rio de Janeiro, Rua Frei Caneca, 94 Centro, 20211-040 Rio de Janeiro, RJ, Brazil

Correspondence should be addressed to Israel Felzenszwalb; moc.liamg@nezlef.jreu

Received 15 August 2016; Revised 12 December 2016; Accepted 26 December 2016; Published 19 January 2017

Academic Editor: Yanzhu Zhu

Copyright © 2017 Carlos F. Araujo-Lima et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Sclerosing agents as zinc gluconate-based chemical sterilants (Infertile®) are used for chemical castration. This solution is injected into the animal testis, but there are not enough evidences of its safety profiles for the receivers. The present work aimed to establish the pharmacokinetics and toxicological activity of Infertile, using in vitro and in silico approaches. The evaluation at the endpoint showed effects in a dose-dependent manner. Since necrosis is potentially carcinogenic, the possible cell death mechanism could be apoptosis. Our data suggested that Infertile at 60 mM presented risk for animal health. Even though Infertile is a licensed product by the Brazilian Ministry of Agriculture, Livestock and Supply, it presented a high mutagenic potential. We suggest that the optimal dose must be less than 6 mM, once, at this concentration, no mutagenicity or genotoxicity was observed.