Lycium barbarum Polysaccharides Protect Rat Corneal Epithelial Cells against Ultraviolet B-Induced Apoptosis by Attenuating the Mitochondrial Pathway and Inhibiting JNK Phosphorylation

Figure 6

LBPs inhibit UVB-induced upregulation of Bax and caspase-3 and downregulation of Bcl-2 in RCE cells. (a, b) Relative mRNA expression changes of Bax and Bcl-2 in UVB−/LBPs−, UVB+/LBPs−, and UVB+/LBPs+ groups. (c) Fold changes in the Bax/Bcl-2 ratio in each group. (d) Relative mRNA expression changes of caspase-3 in each group. (e) Changes in the protein levels of caspase-3 were confirmed by Western blotting. GAPDH was used as an endogenous control protein. (f) Fold changes in the relative optical intensities of caspase-3/GAPDH in each group. and compared with the UVB−/LBPs− group; and compared with the UVB+/LBPs− group; in each group.

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