BioMed Research International / 2017 / Article / Fig 4

Research Article

Decreased Plasma COMP and Increased Plasma CTX-II Levels in a Chinese Pseudoachondroplasia Family with Novel COMP Mutation

Figure 4

Comparison of processes of secretion of wild type and mutant COMP. HEK-293 cells were transfected with expressed plasmids of wild type COMP, mutant COMP, and empty vector, respectively, and for immunofluorescence and Western blot analysis. (a) Western blot after transfection of 12 hours and 24 hours showed the levels of mutant COMP in cells were more than wild type COMP. (b) Immunofluorescence after transfection of 24 hours showed that wild type COMP homogenously expressed in cytoplasm and mutant protein irregularly accumulated inside the cell membrane; .

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