Fabrication and In Vitro Study of Tissue-Engineered Cartilage Scaffold Derived from Wharton’s Jelly Extracellular Matrix

Table 1

Primer sequences of target genes used for RT-qPCR.

Target gene	Primer sequence	NCBI accession number
GAPDH	F: 5′-CAAGAAGGTGGTGAAGCAGG-3′; R: 5′-CACTGTTGAAGTCGCAGGAG-3′	NM_001082253.1
Collagen I; (col1a2)	F: 5′-GCCACCTGCCAGTCTTTACA-3′; R: 5′-CCATCATCACCATCTCTGCCT-3′	NM_001195668.1
Collagen II; (col2a1)	F: 5′-CACGCTCAAGTCCCTCAACA-3′; R: 5′-TCTATCCAGTAGTCACCGCTCT-3′	XM_002723438.1
Collagen X; (col10a1)	F: 5′-CCACCAGGACAAGCAGTCAT-3′; R: 5′-CACTAACAAGAGGCATCCCG-3′	XM_002714724.1
Sox-9	F: 5′-GCGGAGGAAGTCGGTGAAGAAT-3′; R: 5′-AAGATGGCGTTGGGCGAGAT-3′	XM_002719499
Aggrecan	F: 5′-GGAGGAGCAGGAGTTTGTCAA-3′; R: 5′-TGTCCATCCGACCAGCGAAA-3′	XM_002723376.1

Sox-9, (sex determining region Y)-box 9.