Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study

Figure 4

Images obtained by differential interference contrast (DIC) microscopy for initial erythrocytes and erythrocytes after hypoosmotic dialysis with subsequent sealing of the cells in isotonic medium. Initial native erythrocytes (a). Erythrocytes after procedures of hypoosmotic dialysis, sealing, and additional 2 h relaxation of sealed cells (37°C) carried out in the absence (b, c, d) or presence (e, f, g) of ADH (10 IU/ml of RBCs suspension), respectively. The scale bar corresponds to 10 µm. The cells were fixed by 2.5% glutaraldehyde in PBS. Microscope: Zeiss Cell Observer Z.1, objective 100x 1.3 NA, camera QuantEm 512sc. Magnification of 1600x.

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