Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study

Figure 7

Ethanol oxidation by alcocytes in vitro at various concentrations of pyruvate and NAD⁺. (a) Alcocytes were incubated in the medium containing 60 mM pyruvate (■) or without pyruvate (○). The initial rates of ethanol oxidation in the absence and presence of pyruvate were 1.8 and 30 mmol/·h, respectively. Parameters of the mathematical model (lines) were as follows: 170 nkatals/ml for ADH, 43 nkatals/ml for ALD, and 173 nkatals/ml for LDH; hematocrit of 28%; and 16 mM glucose. (b) Alcocytes were produced in the absence (○) or presence (■) of NAD⁺ in the outer dialysis circuit. The initial rates of ethanol oxidation were 2.5 ± 0.4 and 7.3 ± 1.2 mmol/·h, in the absence and presence of NAD⁺, respectively. Parameters of the mathematical model (lines) were as follows: 150−167 nkatals/ml for ADH, 25−33 nkatals/ml for ALD, and 100 nkatals/ml for LDH; hematocrit of 25−27%; and 43 µM NAD⁺ (■) or 9.5 µM NAD⁺ (○). (c) Alcocytes were produced in the presence of 100 µM NAD⁺ (○) or 100 µM NAD⁺ plus 100 µM NADH (■) in the outer dialysis circuit. Initial rates of ethanol oxidation were and  mmol/·h, respectively. Parameters of the mathematical model were as follows: 150 nkatals/ml for ADH, 25 nkatals/ml for ALD, 100 nkatals/ml for LDH, and hematocrit of 25–27%. Typical experiments are presented.

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