Magnetic Separation Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Various Types of Matrices: A Review

Table 3

Magnetic separation methods used for the detection of Mycobacterium avium subsp. paratuberculosis cells in blood.

Method	Type of beads	Coating ligand	Type of sample	Artificially contaminated	Preparation of sample	Initial volume of sample [mL]	Limit of Detection [MAP cells/mL]	Initial number of MAP [PFU/mL]	Capture efficiency [%]	Reference
PMS	MyOne Tosylactivated Dynabeads	Biotinylated peptides (aMp3, aMptD)	Sheep	Yes	10-fold dilution of sample in modified 7H9 Media Plus + 5 µL aMp3 and 5 µL aMptD beads	1	10	3.5 × 10^1	92	[39]
			Horse	Yes		1	n. a.	3.5 × 10^1	92
			Bovine	No		1	n. a.	n. a.	92

Whole blood or isolated buffer coat layer; MAP = Mycobacterium avium subsp. paratuberculosis. n. a. = information not available; PFU = plaque-forming units; PMS = peptide-mediated magnetic separation.