Renoprotective Effects of Aldose Reductase Inhibitor Epalrestat against High Glucose-Induced Cellular Injury

Figure 6

Assessment of relative mitochondrial membrane potential in NRK-52E cells by JC-1 staining. (a) Bar graph showing fluorescence values after 24 h of high glucose exposure with and without 1 µM epalrestat using JC-1 staining. Values were expressed as percentage Mean ± SEM of J-aggregate (excitation/emission 488/529) to monomer (excitation/emission 488/590) ratio compared to control. compared to control. (b) Representative JC-1 stained images of NRK-52E cells treated with high glucose and osmotic control media in the presence or absence of 1 µM epalrestat visualized under 400x magnification using fluorescence microscope.

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