Molecular Cloning, Expression Profiling, and Marker Validation of the Chicken Myoz3 Gene

Table 3

Primers information.

Forward (5′-3′)	Reverse (5′-3′)	Product length (bp)	Annealing temperature (°C)	Amplify target
TCACCCACGTCCACCTTTCTC	TGTCCACTCGTTGGCTGCTC	567	57.6	Exon 1
GTGCAGCGTGCTCCAACAT	GGCATCTGTGGGACAAGGAG	577	58	Exon 2 3
GCAGCAAGAGGAAAGCCAGTG	GGATGGGTGGATGGAAAGATG	359	61.2	Exon 4
TTCCAGACCCGCTTTATCCA	ACGAGACCGCTAGGTTGACTT	372	61	Exon 5

Primers used to detection SNPs of Myoz3 gene.