Retrograde Activation of the Extrinsic Apoptotic Pathway in Spinal-Projecting Neurons after a Complete Spinal Cord Injury in Lampreys

Figure 5

Photomicrographs of dorsal views of whole-mounted brains of larval sea lampreys showing colocalization of cytochrome c and complex Vα immunoreactivities (arrows) in identifiable reticulospinal neurons of control animals (a) and animals 2 (b) and 4 (c) weeks after a complete SCI. Rostral is to the top and the ventricle to the left in all figures. (d) Western blot showing that the anti-cytochrome c antibody recognizes a single band of the expected molecular weight for cytochrome c (about 13 kDa). B3 and B4: Müller cells of the bulbar region; Cc: cytochrome c; Mth: Mauthner cell. Scale bars: 20 μm in (a) and (b) and 15 μm in (c).

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