Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes

Figure 1

Assessment of TPD52 family expression in vivo and in vitro. (a) Immunohistochemistry. A 12-week-old rat was sacrificed and the tibias were removed and subjected to immunohistochemistry for TPD52 and TPD54. Bar, 100 μm. (b) Expression of TPD52 and TPD54 proteins in mouse cell lines. MC3T3-E1, ATDC5, and RAW264.7 cells were cultured in the absence (−) or presence (+) of a differentiation-inducing reagent (see Materials and Methods) and were subjected to western blot analysis for TPD52, TPD54, and GAPDH. (c) Expression of TPD52 and TPD54 genes in mouse cell lines. MC3T3-E1, ATDC5, and RAW264.7 cells were cultured in the absence (open box) or presence (closed box) of a differentiation-inducing reagent (see Materials and Methods) and were subjected to RT-qPCR for TDP52 and TPD54. The value of the uninduced cells was designated as “1,” and relative values are shown. Data are shown as the mean with the standard deviation of three sets of cultures. versus the control.

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