Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes

Figure 2

Analysis of the overexpression or knock-down of TPD52 and TPD54 in MC3T3-E1 cells. The pCMV-HA control (control vector), HA-tagged TPD52 and TPD54 overexpression vectors (a to e) or control, and TPD52 and TPD54 siRNAs (f to j) were transfected into MC3T3-E1 cells, and the cells were subjected to DNA (a and f), ALPase activity (b and g), and Ca deposition (c and h) measurements, as well as RT-qPCR for ALPase (d and i) and osteocalcin (e and j). Data are shown as the mean with the standard deviation of three sets of cultures. versus the control.