Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes

Figure 3

The overexpression and knock-down of TPD52 and TPD54 in ATDC5 cells. The pCMV-HA empty vector (Control), HA-tagged TPD52 and TPD54 overexpression vectors (a) or control, and TPD52 and TPD54 siRNA (b) were transfected into ATDC5 cells. After 48 h, the total proteins of the transfected and untransfected cells (untransfected) were purified and subjected to western blot analysis for TPD52, TPD54, and GAPDH (an internal control).

(a)

(b)