Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes

Figure 4

Analysis of the overexpression of TPD52 and TPD54 in ATDC5 cells. The pCMV-HA control (control vector) and HA-tagged TPD52 and TPD54 overexpression vectors were transfected into ATDC5 cells, and the cells were subjected to DNA (a), GAG (b), ALPase activity (c), and Ca deposition (d) measurements, as well as RT-qPCR for type II collagen (e), aggrecan core protein (f), ALPase (g), and type X collagen (h). For RT-qPCR, the value of the control was designated as “1,” and relative values are shown. Data are shown as the mean with the standard deviation of three sets of cultures. versus the control.