Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes

Figure 6

Cytohistochemical analysis of TPD52 and TPD54 overexpression and knock-down in ATDC5 cells. The pCMV-HA overexpression vectors (OE) and siRNAs (KD) for TPD52 and TPD54 were transfected into ATDC5 cells. After 7 days, the cells were subjected to ALPase (ALP), crystal violet (CV), and toluidine blue (TB) staining. Bar, 1 mm.