Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3K/Akt/GSK-3β/β-Catenin Signaling Pathway

Figure 4

Effects of mechanical stress on PI3K/Akt/GSK-3β/β-catenin signaling pathway. BMSCs were stimulated with different levels of mechanical stress for 30 min. (a) Expression levels of PI3K/Akt/GSK-3β/β-catenin signaling proteins were detected by Western blot. (b–d) Quantitative analysis. versus control group; versus small-magnitude stress group. Each value was presented as mean ± SD of three separate experiments and data of the treatment group was expressed as fold change versus that of control group (labeled as “1.00”). S represented small-magnitude stress; L represented large-magnitude stress.

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