Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3K/Akt/GSK-3β/β-Catenin Signaling Pathway

Figure 5

Effects of activation of β-catenin on BMSCs differentiation. (a) Cells were incubated with or without 10 mM LiCl for 24 h. β-Catenin in BMSCs was detected by Western blot. Cells were cultured in adipogenic medium for 7 days with or without LiCl. Expression levels of Runx2, Collagen I, C/EBPα, and PPARγ were detected by Western blot. (b) Quantitative analysis. versus control group. Each value was presented as mean ± SD of three separate experiments and data of the treatment group was expressed as fold change versus that of control group (labeled as “1.00”).

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