Mechanical Stress Regulates Osteogenesis and Adipogenesis of Rat Mesenchymal Stem Cells through PI3K/Akt/GSK-3β/β-Catenin Signaling Pathway

Figure 6

Activation of PI3K/Akt/GSK-3β/β-catenin signaling by the treatment with LY294002 and mechanical stress. MSCs were exposed to mechanical stress (0.15 Hz × 8 cm) for 30 min in the presence or absence of LY294002. (a) Western blot analysis of the protein levels of p-Akt, GSK-3β, and β-catenin. (b–d) Quantitative analysis. versus control group; versus stress without inhibitor group. Each value was presented as mean ± SD of three separate experiments and data of the treatment group was expressed as fold change versus that of control group (labeled as “1.00”).

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