BioMed Research International / 2017 / Article / Tab 1

Review Article

Do miRNAs Play a Role in Fetal Growth Restriction? A Fresh Look to a Busy Corner

Table 1

Summary of relevant information from selected studies.

Authors, yearType of studyEnrolled populationMethods of analysisSamplesMain findings

Cindrova-Davies et al., 2013 [8]Case-control6 FGR, 6 PE-AD, 7 PE-ND, 7 HP (controls)RT-qPCRPlacentasExpression of miR-21 was significantly upregulated in PE-AD and FGR cases, but not in PE-ND cases

Guo et al., 2013 [9]Case-control26 FGR, 16 PE, 29 HP (controls)RT-qPCRPlacentasDownregulation of miR-149 was shown in PE cases; miR-194 was significantly downregulated in FGR and PE cases

Hromadnikova et al., 2015 [10]Case-control35 GH, 80 PE, 35 FGR, 20 HP (controls)RT-qPCRPlacentas(i) Upregulation of miR-499a-5p in PE, GH, FGR cases;
(ii) upregulation of miR-1-3p FGR-AD in PE cases delivering after 34 weeks; 
(iii) downregulation of miR-26a-5p, miR-103a-3p, miR-145-5p in PE and FGR cases requiring delivery before 34 weeks;  
(iv) downregulation of miR-16-5p, miR-100-5p, miR-122-5p, miR-125b-5p, miR-126-3p, miR-143-3p, miR-195-5p, miR-199a-5p, miR-221-3p, miR-342-3p, miR-574-3p in FGR cases requiring delivery before 34 weeks

Huang et al., 2013 [11]Case-control25 FGR, 25 HP (controls)RT-qPCRPlacentasUpregulation of miR-424

Tang et al., 2013 [13]Case-control21 FGR, 34 HP (controls)RT-qPCRPlacentasHigh expression level of miR-141 in FGR cases

Donker et al., 2012 [14]Case-control14 FGR, 14 HP (controls)RT-qPCRPlacentasHypoxic stress does not affect C19MC miRNA expression, except for downregulation of miR-500c-3p

Higasijima et al., 2013 [15]Two-step case-controlFirst step: 45 FGR, 50 HP (controls)RT-qPCRPlacentas(i) miR-518b, miR-1323, miR-516b, miR-515-5p, miR-520h, miR-519d, miR-526b were significantly lower in FGR placentas than in controls;  
(ii) no differences in miR-516a-5p, miR-525-5p, miR-520a-5p levels
Second step: 10 FGR, 10 HP (controls)RT-qPCRMaternal plasma/serumNo differences were found in miR-518b, hsa-miR-1323, hsa-miR-520h, hsa-miR-519d levels between FGR cases and controls

Wang et al., 2014 [16]Case-control30 FGR, 30 LGA, 30 NGA (controls)RT-qPCRPlacentasDecreased expression of miR-518b and increased expression of miR-519a in FGR cases with respect to NGA and LGA cases

Hromadnikova et al., 2015 [17]Case-control21 GH, 63 PE, 36 FGR, 42 HP (controls)RT-qPCRPlacentas(i) Downregulation of miR-517-5p, miR-519d, miR-520a-5p, miR-525 in GH cases;  
(ii) downregulation of miR-517-5p, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, miR-525 in FGR cases;  
(iii) downregulation of miR-515-5p, miR-517-5p, miR-518b, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, miR-520h, miR-524-5p, miR-525, miR-526a in PE cases

Hromadnikova et al., 2012 [44]Case-control16 PE, 11 FGR, 5 PE and FGR, 7 normal pregnancies with later onset of PE and/or FGR, 10 NP (controls), 50 HP (controls)RT-qPCRMaternal plasma/serum(i) No differences were found in miR-516-5p, miR-517, miR-518b, miR-520a, miR-520h, miR-525, miR-526a concentrations between pathologic and normal pregnancies;  
(ii) significant elevation of miR-516-5p, miR-517, miR-518b, miR-520a, miR-520h, miR-525, miR-526a levels during early gestation in pregnancies with later onset of PE and/or FGR

Hromadnikova et al., 2013 [45]Case-control63 PE, 27 FGR, 23 GH, 55 HP (controls)RT-qPCRMaternal plasma/serum(i) Plasmatic levels of miR-516-5p, miR-517, miR-520a, miR-525, miR-526a did not differ between FGR, GH, and HP patients;  
(ii) increased plasmatic levels and high expression of miR-516-5p, miR-517, miR-520a, miR-525, miR-526a were found in PE patients

Hromadnikova et al., 2016 [46]Case-control39 GH, 68 PE, 33 FGR, 20 HP (controls)RT-qPCRMaternal plasma/serum(i) Downregulation of miR-100-5p, miR-125b-5p, miR-199a-5p in GH, PE, FGR cases compared with controls;  
(ii) downregulation of miR-17-5p, miR-146a-5p, miR-221-3p, miR-574-3p in FGR cases;  
(iii) downregulation of miR-100-5p, miR-125b-5p in PE cases;  
(iv) downregulation of miR-100-5p, miR-125b-5p, miR-146a-5p, miR-199a-5p, miR-221-3p, miR-574-3p in FGR cases;  
(v) downregulation of miR-100-5p, miR-125b-5p, miR-199a-5p in patients with GH

Mouillet et al., 2010 [47]Case-control14 FGR, 14 HP (controls)RT-qPCRMaternal plasma/serum(i) No differences were found in the level of individual miRNA (miR-27a, miR-30d, miR-141, miR-200c, miR-424, miR-205 and miR-451, miR-491, miR-517a, miR-518b, miR-518e, miR-524) in FGR and HP cases;  
(ii) When considered as a group, the level of all tested miRNA species was elevated in plasma samples from women with FGR compared to controls

HP: healthy pregnancies; FGR: fetal growth restriction; PE: preeclampsia; -AD: abnormal fetal-Doppler; -ND: normal fetal-Doppler; GH: gestational hypertension; LGA: large for gestational age; NGA: normal for gestational age; NP: nonpregnant; RT-qPCR: real-time quantitative reverse transcription polymerase chain reaction.