Hidden IgG Antibodies to the Tumor-Associated Thomsen-Friedenreich Antigen in Gastric Cancer Patients: Lectin Reactivity, Avidity, and Clinical Relevance

Figure 3

The effect of IgG-depleted serum on the level of anti-TF HAb in cancer patients and controls. The purified tIgG of cancer patients () and controls () was incubated with autologic IgG-depleted serum diluted from 1 : 10 to 1 : 100 for 15 min at 25°C. The level of anti-TF hidden antibodies was determined as described in Material and Methods; the mean value of HAb levels (OD) before and after addition of IgG-depleted serum dilutions is presented.