Histopathological and Reproductive Evaluation in Male Rats Fed Jatropha curcas Seed Cake with or without Alkaline Hydrolysis and Subjected to Heat Treatment
Table 3
Reproductive parameters of untreated female rats mated with male rats that received a diet of 5% of Jatropha curcas seed cake subjected to heat treatment and different methods of detoxification or standard feed (control) during 60 days.
Parameters
Control
J. curcas nonhydrolyzed
J. curcas hydrolyzed
I
Washed with ethanol (II)
Washed with water (III)
Unwashed (IV)
Washed with ethanol (V)
Washed with water (VI)
Unwashed (VII)
Pregnant total
9/10
8/10
8/10
10/10
9/10
8/10
9/10
Maternal body weight (g
360 ± 14.38
371.12 ± 11.31
377 ± 7.94
363.00 ± 8.03
351.33 ± 9.44
366.75 ± 14.15
369.44 ± 8.51
Pregnant uterus weight (g
61.57 ± 5.68
56.58 ± 5.25
62.01 ± 5.73
51.6 ± 6.24
61.10 ± 5.16
58.65 ± 6.61
61.89 ± 5.77
Number of corpora lute
13.66 ± 0.50
14.00 ± 0.60
13.25 ± 0.67
13.00 ± 0.65
13.77 ± 0.61
13.62 ± 0.56
13.55 ± 0.70
Number of implantation
13.33 ± 0.55
12.50 ± 0.59
12.25 ± 0.70
11.30 ± 0.65
12.44 ± 1.04
12.37 ± 0.56
12.66 ± 0.80
Number of reabsorption site
0.44 ± 0.24 (3)
0.87 ± 0.47 (4)
0.50 ± 0.18 (4)
1.90 ± 0.67 (8)
0.88 ± 0.51 (3)
0.75 ± 0.25 (5)
0.77 ± 0.43 (3)
Placental weight (g
0.40 ± 0.02
0.43 ± 0.04
0.44 ± 0.03
0.51 ± 0.0
0.46 ± 0.01
0.42 ± 0.02
0.45 ± 0.02
Litter weight (g
35.24 ± 4.89
34.29 ± 4.44
35.13 ± 4.56
29.03 ± 4.73
34.63 ± 4.27
35.72 ± 7.27
35.06 ± 5.45
Body weight of fetuses (g
2.70 ± 0.21
2.98 ± 0.18
2.91 ± 0.15
3.11 ± 0.25
2.94 ± 0.19
2.91 ± 0.36
2.92 ± 0.27
Crown-rump length (cm
4.90 ± 0.21
4.76 ± 0.12
4.75 ± 0.11
4.65 ± 0.16
4.88 ± 0.11
4.84 ± 0.30
4.90 ± 0.13
Number of live fetuse
12.88 ± 0.73
11.62 ± 0.82
11.75 ± 0.79
9.20 ± 0.8
11.55 ± 0.83
11.62 ± 0.62
11.89 ± 0.69
Number of dead fetuse
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.20 ± 0.00 (1)
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
Preimplantation loss (%
2.45 ± 1.77 (2)
10.30 ± 3.73 (5)
7.36 ± 2.91 (5)
11.97 ± 5.56 (4)
10.57 ± 5.25 (4)
8.85 ± 3.31 (5)
6.53 ± 3.06 (4)
Postimplantation loss (%
3.82 ± 2.14 (3)
7.29 ± 4.00 (4)
4.50 ± 1.75 (4)
18.17 ± 6.11 (8)
5.85 ± 3.30 (3)
6.17 ± 1.94 (5)
5.47 ± 2.94 (3)
Data shown are mean ± SEM. Values in parentheses indicate the number of litters affected. Where appropriate: test; -Wallis test. compared to the control group.
