The Interaction of Selectins and PSGL-1 as a Key Component in Thrombus Formation and Cancer Progression

Figure 6

To determine whether any type of selectins may be expressed on cell surface on M35/01 human melanoma cancer cells, flow cytometry (FACSCalibur, Becton Dickinson) was first assessed: (a) Cells were gated based on their CD146-FITC positivity (CD146/MUC18 is an adhesion molecule on melanoma cells [35]) and SSC characteristics (data not shown). These malignant cells showed a substantial E-selectin expression with monoclonal anti-E-selectin antibody but were negative for surface L- and P-selectin. To confirm these results, CLSM (Olympus IX 81 with Fluoview FV 1000) was also used to observe E-selectin (red) and CD146 (green) positivities. (b) IgG-PE and IgG-FITC antibodies were applied to exclude the background staining. Transmission photos (grey) were used to visualize the cells.

(a)

(b)