Polysaccharides from Trichosanthes Fructus via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Table 2

The BBD matrix with four variables and response values for the yield of crude TFP.

Run	(pH)	(enzyme amount, u/g)	(extraction temperature, °C)	(liquid-to-solid ratio, ml/g)	(yield of crude TFP, %)
1	4	4000	45	30	4.51
2	5	4000	45	30	4.99
3	4	6000	45	30	5.58
4	5	6000	45	30	5.33
5	4.5	5000	40	20	4.2
6	4.5	5000	50	20	4.29
7	4.5	5000	40	40	5.5
8	4.5	5000	50	40	5.43
9	4	5000	45	20	3.46
10	5	5000	45	20	4.11
11	4	5000	45	40	4.6
12	5	5000	45	40	4.53
13	4.5	4000	40	30	5.44
14	4.5	6000	40	30	5.5
15	4.5	4000	50	30	5.7
16	4.5	6000	50	30	6.24
17	4	5000	40	30	4.32
18	5	5000	40	30	5
19	4	5000	50	30	5.32
20	5	5000	50	30	5.11
21	4.5	4000	45	20	4.1
22	4.5	6000	45	20	4.58
23	4.5	4000	45	40	5.71
24	4.5	6000	45	40	5.92
25	4.5	5000	45	30	6.7
26	4.5	5000	45	30	6.56
27	4.5	5000	45	30	6.43
28	4.5	5000	45	30	6.63
29	4.5	5000	45	30	6.6
Optimal conditions	4.49	5373.44	46.06	32.25	6.71