Txndc9 Is Required for Meiotic Maturation of Mouse Oocytes

Figure 1

Expression and localization of Txndc9 during mouse oocyte meiosis maturation. (a) The mRNA level of Txndc9 during mouse oocyte meiosis maturation, GAPDH as the reference gene. (b) Txndc9 protein expression was detected by Western blot in GV, GVBD, MI, ATI, and MII stages and the relative protein level (Txndc9/β-actin) was analyzed and shown in (c). In (a) and (c), data are means ± SD from three independent experiments. , . (d) Subcellular localization of Txndc9 during mouse oocyte maturation in GV, GVBD, MI, ATI, and MII stages. Green: Txndc9; Blue: DAPI. (e) Oocytes were costained with anti-Txndc9 and anti-α-tubulin antibodies in AnaI stage. Green: α-tubulin; red: Txndc9; blue: DAPI.

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