Angelica sinensis Suppresses Body Weight Gain and Alters Expression of the FTO Gene in High-Fat-Diet Induced Obese Mice

Table 2

Information of primer pairs used in this study.

Primer name	Primer sequence (5′-3′)	Size (bp)	Tm (°C)
QPCR
FTO-Q-1F	GAGTTCTATCAGCAGTGG	163	55.0
FTO-Q-1R	GCACATCTTTGCCTTGGA
GAPDH-1F	GGTGAAGGTCGGTGTGAACG	233	55.0
GAPDH-1R	CTCGCTCCTGGAAGATGGTG
ACTB-1F	CGTTGACATCCGTAAAGACC	281	55.0
ACTB-1R	AACAGTCCGCCTAGAAGCAC
18S rRNA-1F	AGGGGAGAGCGGGTAAGAGA	241	55.0
18S rRNA-1R	GGACAGGACTAGGCGGAAC
BPS
FTO-B1F	TAGTTGATTTTGTTTGAAGAGGAAGA	671	65.2
FTO-B1R	TCCTACTCACTATCAACAATTCCTAA
FTO-B2F	GGGTTGAAGAGGTGGTTTAGTAGTTA	496	57.5
FTO-B2R	ACAATCTCACTCAATCCACTTACATCT